Regeneration of plants from callus cultures of roots induced by Agrobacterium rhizogenes on Alhagi pseudoalhagi
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منابع مشابه
Somatic Embryogenesis and Plantlet Regeneration from Hairy Roots Transformed by Agrobacterium rhizogenes in Panax quinquefolium L
Here, efficient plantlet regeneration via somatic embryogenesis from Panax quinquefolium hairy roots was reported. Callus development was optimal on MS medium containing 2,4-dichlorophenoxyacetic acid (1 mg l) and -naphthalene acetic acid (1 mg l). After a 4-week growth, calli were transferred onto MS medium with different combinations of plant growth regulators (PGRs). The highest embryogenesi...
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Composite plants consisting of a wild-type shoot and a transgenic root are frequently used for functional genomics in legume research. Although transformation of roots using Agrobacterium rhizogenes leads to morphologically normal roots, the question arises as to whether such roots interact with arbuscular mycorrhizal (AM) fungi in the same way as wild-type roots. To address this question, root...
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Plants are the important sources of drugs. Secondary metabolites are responsible for therapeutic propertiesin plants. Three compounds including (-)-hyoscyamine, its racemate atropine, and scopolamine (hyoscine) are the most famous tropane alkaloids in the Solanaceae familly. Nowadays, attempts to developthese alkaloids in biotechnological procedures which are principally based on the hairy root...
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In vitro raised shoots of Plumbago rosea L. were infected with A4 strain of Agrobacterium rhizogenes to initiate hairy root formation, which produced 3.0±0.33 hairy roots per incision on explants in 20 d incubation. Southern blot analysis confirmed the integration of T-DNA into the genome of the roots. The hairy roots were cultured on MS agar medium supplemented with 2.0 mg/L BAP to induce the ...
متن کاملSmall RNAs Derived from the T-DNA of Agrobacterium rhizogenes in Hairy Roots of Phaseolus vulgaris
Agrobacterium rhizogenes is a pathogenic bacteria that causes hairy root disease by transferring bacterial DNA into the plant genome. It is an essential tool for industry and research due to its capacity to produce genetically modified roots and whole organisms. Here, we identified and characterized small RNAs generated from the transfer DNA (T-DNA) of A. rhizogenes in hairy roots of common bea...
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ژورنال
عنوان ژورنال: Cell Research
سال: 2001
ISSN: 1001-0602,1748-7838
DOI: 10.1038/sj.cr.7290097